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ТИПИРОВАНИЕ ТКАНЕЙ МЕТОДАМИ ПЦР И КАПИЛЛЯРНОГО ЭЛЕКТРОФОРЕЗА: РЕАЛЬНОСТЬ, ВОПРОСЫ И ПЕРСПЕКТИВЫ

https://doi.org/10.19048/2411-8729-2017-3-1-48-58

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Аннотация

Целью текущего обзора является раскрытие новых перспектив для РНК- и ДНК-анализа в рутинной практике судебной биологии. В обзоре рассмотрены современные тканевые маркеры, основанные на матричных РНК (мРНК), методы их тестирования и подходы для совмещения РНК-анализа со стандартными протоколами исследования судебного материала. Исходя из практического опыта зарубежных исследователей, новые маркеры вполне совместимы с классическими протоколами, включая использование методов ПЦР и капиллярного электрофореза, и могут быть применены после постановки иммунологических тестов, что усиливает чувствительность и не требует принципиального отказа от их использования. На сегодняшний день сформированы относительно готовые мультиплексные тканевые наборы, подготовленные для коммерческих реализаций в скором будущем. Популяционное генетическое разнообразие в экспрессии генов определяет возможность разработки отечественных панелей мРНК-маркеров на базе существующих зарубежных панелей.

Об авторе

А. С. Бавыкин
ООО «Ниармедик плюс»
Россия
к.м.н., LLC «Nearmedic Plus› 125252, г. Москва


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Для цитирования:


Бавыкин А.С. ТИПИРОВАНИЕ ТКАНЕЙ МЕТОДАМИ ПЦР И КАПИЛЛЯРНОГО ЭЛЕКТРОФОРЕЗА: РЕАЛЬНОСТЬ, ВОПРОСЫ И ПЕРСПЕКТИВЫ. Судебная медицина. 2017;3(1):48-58. https://doi.org/10.19048/2411-8729-2017-3-1-48-58

For citation:


Bavykin A.S. TISSUE TYPING BY MEANS OF PCR AND CAPILLARY ELECTROPHORESIS: NEW ASPECTS, REALITY AND ISSUES. Russian Journal of Forensic Medicine. 2017;3(1):48-58. (In Russ.) https://doi.org/10.19048/2411-8729-2017-3-1-48-58

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ISSN 2411-8729 (Print)
ISSN 2409-4161 (Online)